Original research

Diagnostic value of Xpert MTB/RIF assay on pleural tissue obtained via closed pleural biopsy among persons with presumptive tuberculous pleuritis

SD Yoo, G Abebe, JE Yoo, D Regassa, M Bezabih

Abstract


Background. Tuberculous pleuritis (TBP) is a common extrapulmonary tuberculosis that contributes to the tuberculosis burden. Xpert MTB/RIF assay is a promising method for rapid diagnosis of TBP. The diagnostic value of Xpert MTB/RIF assay in pleural tissue obtained via closed pleural biopsy among sputum acid-fast bacilli (AFB) smear-negative persons is not well studied. 

Objective. To evaluate the diagnostic value of Xpert MTB/RIF assay on diagnosis of TB in pleural tissue obtained via blind closed pleural biopsy. 

Methods. Closed pleural biopsy using Cope needle was performed on adult patients who presented with lymphocyte predominant exudative pleural effusion. Xpert MTB/RIF assay was performed in parallel to pathology and mycobacterial culture of the pleural tissue specimen to determine its sensitivity and specificity. Final clinical diagnosis of TBP was determined by improvement in 2-months follow-up of antituberculous treatment. 

Results. A total of 33 patients were included in the study. The median (interquartile range (IQR)) age was 27 (25 - 42) years. The sensitivity and specificity of Xpert MTB/RIF assay was 30% and 100% compared with Mycobacterium tuberculosis culture as the gold standard, and 20% and 95.7% compared with histopathology as the gold standard. 

Conclusion. Xpert MTB/RIF assay in pleural tissue obtained by closed pleural biopsy did not increase diagnostic yield, but it shortens time for diagnosis compared with conventional methods.


Authors' affiliations

SD Yoo, Department of Internal Medicine, Jimma University Institute of Health, Jimma University, Jimma, Ethiopia

G Abebe, Department of Microbiology, Jimma University Institute of Health, Jimma University, Jimma, Ethiopia

JE Yoo, Department of Environmental Health, Jimma University Institute of Health, Jimma University, Jimma, Ethiopia

D Regassa, Department of Epidemiology, Jimma University Institute of Health, Jimma University, Jimma, Ethiopia

M Bezabih, Department of Pathology, Jimma University Institute of Health, Jimma University, Jimma, Ethiopia

Full Text:

PDF (163KB)

Cite this article

African Journal of Thoracic and Critical Care Medicine 2021;27(1):10. DOI:10.7196/AJTCCM.2021.v27i1.120

Article History

Date submitted: 2021-03-09
Date published: 2021-03-09

Article Views

Abstract views: 565
Full text views: 261

Refbacks

  • There are currently no refbacks.



African Journal of Thoracic and Critical Care Medicine| Online ISSN: 2617-0205

This journal is protected by a Creative Commons Attribution - NonCommercial Works License (CC BY-NC 4.0) | Read our privacy policy.

Our Journals: South African Medical Journal | African Journal of Health Professions Education | South African Journal of Bioethics and Law | South African Journal of Child Health | Southern African Journal of Critical Care | African Journal of Thoracic and Critical Care MedicineSouth African Journal of Obstetrics and Gynaecology |